Bioassay

bogorlab provides toxicology tests such as LD50 and LC50

LD (Lethal Dose) 50 is defined as the amount of a material, given all at once, which causes the death of 50% of a group or population of test animals. It is usually expressed as the amount of chemical administered (e.g., milligrams) per 100 grams (for smaller animals) or per kilogram (for bigger test subjects) of the bodyweight of the test animal. The LD50 can be found for any route of entry or administration but dermal (applied to the skin) and oral (given by mouth) administration methods are the most common. The LD50 is one way to measure the short-term poisoning potential (acute toxicity) of a material. Usually, it uses 96 hours on the test or according to requirement. We use mice (Mus musculus) as test animals. The objective of this test was to determine the acute oral median lethal of the test material when administered as a single dose to mice. The test was conducted in general in accordance with the ‘Health Effect Test Guidelines OPPTS 870.1100. Acute Toxicity Testing – Acute Oral Toxicity EPA 712-C-98-190’ (EPA 1998b.).

Meanwhile, LC stands for ‘Lethal Concentration’. LC values usually refer to the concentration of a chemical in air but in environmental studies it can also mean the concentration of a chemical in water. The concentration of the chemical in water that kills 50% of the test animals in a given time is the LC50 value. Bogorlab uses Penaeus monodon as animal tests. Penaeus monodon is standard test organism that can be exposed to man-made chemicals during manufacturing, used or disposal to find LC50. The test is conducted in general accordance with the ‘Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms’ (EPA, 2002).

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